Asian Journal of Bio Science

R. Anand ¹, R. Aswini ¹, V. H. Mary Jiji ¹, V. Keerthana ¹

Affiliations
1 Postgraduate and Research Department of Microbiology, Dr.N.G.P.Arts and Science College, Coimbatore (T.N.), IN

Abstract

Bacillus sps-26 isolated and characterized from dairy effluents was challenged for its ability to produce alkaline proteinase using different temperature and pH as bioparameters with a chemically defined medium containing 5% casein. The maximum enzyme production was achieved at 30.C at pH 10, which was assayed using Genov method. Although moderate enzyme level was obtained at 10.C, it can pave the way for its affordable use in detergent industries.

Keywords

Bacillus sp-26, Alkaline Proteinase, Bioparameters, Tseushida Method, Lowry Method.

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